One feeding protocol

This section is dedicated to the discussion of jellyfish foods and feeding techniques

One feeding protocol

Postby jonathanyhsu » 20 Oct 2012, 00:28

Equipment:
- 2L hatchery cones with stands - http://www.brineshrimpdirect.com/c9/Lar ... -p243.html
- Tetra Whisper 60gal air pump - http://www.amazon.com/Tetra-77854-Whisp ... 0009YJ4NG/
- 100mL of EZ Egg (decapsulated eggs) - http://www.brineshrimpdirect.com/Shell- ... -c202.html
- 125mL of Nannochloropsis algae paste (glycerin free) - http://www.brineshrimpdirect.com/c6/Nan ... r-c34.html
- 125mL of SELCO S.PRESSO - http://www.brineshrimpdirect.com/SELCO-c47.html
- 1 gal of 6% sodium hypochlorite (bleach)
- 25 ppt saltwater

Advance preparation:
- Sterilize the hatcheries by filling them with 2L of water each, and 10mL of 6% sodium hypochlorite solution. Most caps on bleach containers are approximately 10mL, so this equates to 1 capful of bleach. This will come out to be 300ppm sodium hypochlorite in the hatchery. Let sit overnight for several hours and then rinse out thoroughly and dry.

0 hours:
- Fill hatchery with 1.5L of 25 ppt saltwater.
- Thread silicon tubing through evaporation lid, attach silicon tubing to rigid air line and air pump. Turn on air pump.
- Put in 2 spoons of EZ egg (4g or 2g dry weight equivalent) in aerated hatchery water.
- Put in 3 drops of 6% sodium hypochlorite.
- Turn on a 100W incandescent light, or use other heat + light source to raise temperature to 78-80F.

24 hours:
- Drain hatchery with nauplii through a filter (sieve box, net, etc.) to collect nauplii and dispose of hatchery water.
- Rinse nauplii in a 2L beaker with 2L of pure water, let sit for 5 minutes.
- Use pipette or turkey baster to siphon out 1L of water with hatching membranes at top of beaker while nauplii concentrate at bottom of beaker. Use care not to disturb the nauplii and remix them into the water.
- Refill 2L beaker with 1L of pure water and repeat one or two more times.
- Feed any nauplii desired for the day.
- Put remaining nauplii in a new sterilized hatchery with 1.5L of 25-35ppt saltwater. Turn on aeration.
- Put in 1.5mL of Nannochloropsis algae paste
- Put in 5-6 drops of SELCO S.PRESSO

36 hours:
- Put in 1.5mL of Nannochloropsis algae paste
- Put in 5-6 drops of SELCO S.PRESSO

48 hours:
- Drain hatchery with nauplii through a filter (sieve box, net, etc.) to collect nauplii and dispose of hatchery water.
- Rinse nauplii in a 2L beaker with 2L of pure water, let sit for 5 minutes.
- Use pipette or turkey baster to siphon out 1L of water with hatching membranes at top of beaker while nauplii concentrate at bottom of beaker. Use care not to disturb the nauplii and remix them into the water.
- Refill 2L beaker with 1L of pure water and repeat one or two more times.
- Feed any nauplii desired for the day.
- Freeze any remaining nauplii for later use.
jonathanyhsu
 
Posts: 3
Joined: 26 May 2012, 16:11

Re: One feeding protocol

Postby Cubic » 26 Oct 2012, 03:56

Thanks Jonathan

That's a very clear and well written guide to hatching and harvesting artemia nauplii. :thumbup:

Have you had any experience with Ocean Nutrition's Sep-Art artemia cycts?

http://reefbuilders.com/2012/08/07/sepa ... a-cysts-2/

If so, what do you think of them? They seem a very interesting idea but as of yet we haven't had the chance to try them out.
“Have nothing in your homes that you do not know to be useful or believe to be beautiful.”

William Morris.
User avatar
Cubic
Site Admin
 
Posts: 162
Joined: 02 Nov 2011, 17:22

Re: One feeding protocol

Postby Dr. T » 26 Oct 2012, 12:09

Thanks for your post Jonathan! I didn't know there are decupsulated Artemia cysts on sale.

I have two questions.

1. I have never rinsed Artemia naupii with fresh water. Do they survive alright with that?
2. The membranes of Artemia nauplii after they hatch are pain on the arse aren't they? Is that the reason you use fresh water to rinse them so that you can separate them from nauplii?

Cheers
Image
User avatar
Dr. T
 
Posts: 26
Joined: 31 Mar 2012, 06:39

Re: One feeding protocol

Postby nonamethefish » 08 Mar 2014, 00:03

A few notes.

Rinsing BBS in freshwater should be fine-they survive for hours in freshwater tanks anyway.

Brine shrimp nauplii can be stored for several days in the fridge in seawater. I have gone nearly a week with minimal losses. I was not enriching brine shrimp but if I were to try this I would probably enrich them with something they could ingest like the phytoplankton, rinse them well under the sink, and them put them in shallow dishes of seawater in the fridge. Make sure not to knock them over in your fridge! Also, if the nauplii stack more than a couple layers thick they seem to suffocate the ones on the bottom so keep that in mind while storing. However it should be noted that they do take a while to start swimming again when added back into warm water.
nonamethefish
 
Posts: 16
Joined: 14 Jan 2014, 01:06


Return to Feeding Jellyfish

Who is online

Users browsing this forum: No registered users and 2 guests

cron